Agdia announces the commercialisation of a DNA - based assay on their AmplifyRP ® XRT weapons platform for the signal detection of Ralstonia solanacearum race 3 biovar 2 ( RsR3B2 ) .
Ralstonia solanacearum ( Rs ) , formerly known as Pseudomonas solanacearum , have bacterial wilt in numerous crops of economical importance worldwide . It is the causal agentive role of disease include Southern wilt of geranium , Bacterial wilt of tomato , brown rot of potato , Moko disease , Bugtok disease , and Blood Disease of banana .
Ralstonia solanacearum is list as an A2 ( mellow endangerment ) quarantine pathogen in the European and Mediterranean Plant Protection Organization ( EPPO ) . In the United States , Ralstonia solanacearum raceway 3 biovar 2 is a Select Agent due to the possible wallop on the domesticated agricultural industry .
you could geminate the new check with Agdia ’s AmpliFire ® portable isothermal fluorometer .
In 2020 , Ralstonia solanacearum airstream 3 biovar 2 was detected in a U.S. glasshouse for the first time in 16 age . The infestation was traced back to geranium cuttings from an offshore location . Extensive examination and prompt mitigation sweat were employed to successfully eliminate the pathogen .
Agdia ’s new AmplifyRP XRT assay for detection of Ralstonia solanacearum race 3 biovar 2 is based on recombinase polymerase amplification ( RPA ) . This engineering kick upstairs the speedy gain and detection of nucleic acid targets , DNA or RNA , while maintaining a single operating temperature of 39–42 ° one C . The AmplifyRP XRT products achieve sensitiveness and specificity comparable to PCR , while ingest clear advantages over the research lab - base technology .
AmplifyRP XRT products do not necessitate a nucleic Lucy in the sky with diamonds refining step ; unrefined sample extracts are make using a simple extraction polisher and try directly . When paired with Agdia ’s AmpliFire isothermal fluorometer ( design 1 ) , the XRT system is a user - friendly tool that can be implemented in the domain or the lab by force with modified experience in molecular diagnostics . full assay time is less than 30 minutes when used with the AmpliFire as a tangible - time check .
Extensive product validation was conducted to show fitness for purpose . Agdia states their new assay was shield against 62 bacterial culture representative of other races and biovars of Rs in improver to 117 cultures of other relevant bacterial species . No crossbreed - responsiveness was observe , confirming exclusive specificity of the check to RsR3B2 . Additionally , no boniface reactions were observed in validation examination against geranium , tomato , potato , pepper , ginger , banana , or eggplant sample . The young check successfully detected all 19 distinct polish of Rs race 3 , biovar 2 .
For more information : Agdia Inc.52642 County Road 1Elkhart , IN 46514phone 1 - 574 - 264 - 2615fax 1 - 574 - 264 - 2153[email protected]www.agdia.com
